TABLE 1.
Susceptibilities and resistance mechanisms of carbapenemase-producing isolates and their transconjugants
| Isolate no.a | Species | Siteb | MIC (μg/ml) of drugc |
Plasmid size(s) (kb) | Resistance mechanism(s) | Integron | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IPM | MEM | ETP | FOX | TZP | CTX | CAZ | FEP | CIP | AMK | TGC | POL | ||||||
| ZJ18 | K. pneumoniae | ZJ1 | 16 | 8 | 16 | >256 | >256 | 128 | 64 | 16 | >32 | >256 | 1 | 0.5 | 147, 6.4, 4.1, 3.4 | KPC-2, SHV-28, DHA-1, ompK35 loss | |
| ZJ18T | 2 | 1 | 2 | 128 | 64 | 8 | 16 | 4 | 1 | >256 | 0.06 | 0.25 | 147 | KPC-2, DHA-1 | |||
| ZJ70 | K. pneumoniae | ZJ2 | >256 | 256 | >256 | 128 | >256 | >256 | 64 | >256 | 16 | >256 | 0.25 | 0.5 | 42, 5.2, 2.5 | KPC-2, SHV-11, CTX-M-14, ompK35/36 loss | dfrA12-orfF-aadA2 |
| ZJ70T | 16 | 8 | 16 | 32 | >256 | 64 | 32 | 32 | 0.13 | 1 | 0.13 | 0.25 | 42 | KPC-2 | |||
| ZJ71 | K. pneumoniae | ZJ2 | 16 | 16 | 16 | 16 | >256 | 256 | 32 | 128 | 16 | >256 | 0.13 | 1 | 42, 5.2, 2.5 | KPC-2, TEM-1, SHV-11, CTX-M-14, ompK35 loss | dfrA12-orfF-aadA2 |
| ZJ71T | 8 | 4 | 4 | 32 | >256 | 16 | 32 | 16 | 0.25 | 1 | 0.13 | 0.5 | 42 | KPC-2 | |||
| ZJ99 | K. pneumoniae | ZJ1 | 16 | 4 | 8 | 32 | >256 | 128 | 64 | 32 | >32 | 0.5 | 1 | 1 | 90 | KPC-2, CTX-M-3, qnrS1, ompK35 DEd | |
| ZJ99T | 16 | 16 | 8 | 32 | >256 | 256 | 128 | 64 | 2 | 2 | 0.25 | 0.25 | 90 | KPC-2, CTX-M-3, qnrS1 | |||
| ZJ86 | E. coli | ZJ1 | 8 | 8 | 32 | 64 | 256 | 64 | 32 | 32 | >32 | 1 | 0.25 | 0.5 | 120, 23, 7.1, 6.4, 5.9, 5.3, 3.9, 2.9, 2.3, 1.0 | KPC-2, TEM-1, ompC loss | dfrA17-aadA5 |
| ZJ86T | 8 | 8 | 16 | 64 | >256 | 32 | 32 | 16 | 0.25 | 32 | 0.25 | 0.5 | 120 | KPC-2 | |||
| ZJ87 | E. coli | ZJ1 | 8 | 8 | 16 | 64 | 256 | 64 | 32 | 32 | >32 | 1 | 0.25 | 0.5 | 120, 23, 7.1, 5.9, 5.3, 3.9, 2.9, 2.3, 1.0 | KPC-2, TEM-1, ompC loss | dfrA17-aadA5 |
| ZJ87T | 8 | 8 | 16 | 64 | >256 | 32 | 32 | 16 | 0.25 | 1 | 0.25 | 0.25 | 120 | KPC-2 | |||
| FZ47 | K. pneumoniae | FZ | 8 | 2 | 8 | >256 | 64 | 256 | >256 | 128 | 4 | >256 | 1 | 1 | 190, 110, 80 | IMP-8, TEM-1, SHV-11, CTX-M-14, qnrB2, aac(6′)-Ib, ompK35/36 loss | dfrA12-orfF-aadA2 |
| FZ47T | 4 | 1 | 4 | >256 | 8 | 128 | >256 | 64 | 1 | >256 | 0.06 | 0.25 | 190 | IMP-8, TEM-1, CTX-M-14, qnrB2, aac(6′)-Ib | dfrA12-orfF-aadA2 | ||
| FZ49 | K. pneumoniae | FZ | 8 | 2 | 4 | >256 | 128 | >256 | >256 | 256 | 0.5 | >256 | 0.5 | 1 | 190, 110, 80 | IMP-8, TEM-1, SHV-11, CTX-M-14, qnrB2, aac(6′)-Ib, ompK35/36 loss | dfrA12-orfF-aadA2 |
| FZ49T | 4 | 1 | 4 | >256 | 8 | 128 | >256 | 32 | 1 | >256 | 0.06 | 0.25 | 190 | IMP-8, TEM-1, CTX-M-14, qnrB2, aac(6′)-Ib | dfrA12-orfF-aadA2 | ||
| GZ64 | K. pneumoniae | GZ1 | 16 | 8 | 32 | >256 | >256 | 256 | >256 | 128 | 2 | 1 | 0.5 | 0.5 | 140, 49, 11, 5.9, 3 | IMP-4, ompK35/36 loss | IMP-4-orfII |
| GZ64T | 2 | 2 | 4 | 256 | 8 | 128 | 256 | 16 | 0.25 | 1 | 0.06 | 0.5 | 140, 49, 11, 5.9 | IMP-4 | IMP-4-orfII | ||
| WH76 | K. pneumoniae | WH | 2 | 4 | 2 | >256 | 16 | 128 | 256 | 32 | 0.5 | 1 | 0.5 | 1 | 57 | IMP-4, TEM-1, SHV-14, CTX-M-3, qnrS1 | |
| WH76T | 4 | 8 | 2 | >256 | 32 | 256 | >256 | 64 | 2 | 1 | 0.06 | 0.5 | 57 | IMP-4, TEM-1, CTX-M-3, qnrS1 | |||
| WH77 | K. pneumoniae | WH | 2 | 4 | 4 | >256 | 32 | 256 | 256 | 32 | 0.5 | 1 | 0.5 | 1 | 57 | IMP-4, TEM-1, SHV-14, CTX-M-3, qnrS1 | |
| WH77T | 4 | 4 | 4 | >256 | 32 | 256 | >256 | 64 | 2 | 1 | 0.06 | 0.5 | 57 | IMP-4, TEM-1, CTX-M-3, qnrS1 | |||
| SZ62 | C. freundii | SZ | 4 | 4 | 4 | >256 | 256 | 256 | >256 | 64 | 32 | >256 | 1 | 0.5 | 48, 33, 5.5, 5.4, 4.2, 3.3, 2.8, 1.7, 1.2 | IMP-4, TEM-1, CMY-2, qnrS1, aac(6′)-Ib-cr | |
| SZ62T | 4 | 4 | 4 | >256 | 32 | 128 | >256 | 32 | 4 | 1 | 0.13 | 0.25 | 48 | IMP-4, qnrS1 | |||
| SZ63 | C. freundii | SZ | 4 | 4 | 4 | >256 | >256 | >256 | >256 | 128 | 32 | >256 | 1 | 0.5 | 171, 48, 33, 6.8, 5.5, 5.4, 4.2, 3.3, 2.8, 1.7, 1.2 | IMP-4, qnrS1 | dfrA12-orfF-aadA2 |
| SZ63T | 4 | 4 | 4 | >256 | 32 | 256 | >256 | 64 | 2 | >256 | 0.13 | 0.5 | 171, 48 | IMP-4, qnrS1 | dfrA12-orfF-aadA2 | ||
| SZ92 | E. cloacae | SZ | 8 | 8 | 32 | >256 | 128 | >256 | >256 | 128 | >32 | 1 | 1 | 0.5 | 55, 7, 5.9, 5.5, 4.9, 2.5, 1.1 | IMP-4, TEM-1, CTX-M-14, DHA-1, qnrB10/B4-like, aac(6′)-Ib, ompC loss | orfx-aac6′-II, aadA1 |
| RJ94 | E. cloacae | RJ | 8 | 8 | 32 | >256 | 256 | >256 | >256 | 256 | 4 | 0.5 | 1 | 0.5 | 98, 48 | IMP-4, TEM-1, CTX-M-3, ompC loss | PSE-1 |
| WH103 | E. cloacae | WH | 8 | 8 | 32 | >256 | >256 | >256 | >256 | >256 | 32 | 1 | 1 | 0.5 | 118, 51 | IMP-4, TEM-1, CTX-M-3, qnrS1, ompC loss | dfrA12-aadA2 |
| C600 | E. coli | 0.25 | 0.03 | 0.008 | 16 | 1 | 0.13 | 0.5 | 0.06 | 0.25 | 1 | 0.06 | 0.5 | ||||
E. coli C600 was the recipient; T at the end of the isolate number indicates the transconjugant.
FZ, The Affiliated Union Hospital of Fujian Medical University; GZ1, The First Affiliated Hospital of Zhongshan University; RJ, The Affiliated Ruijin Hospital of Shanghai Jiaotong University; SZ, Shenzhen People's Hospital; WH, The Affiliated Tongji Hospital of Huazhong University of Science and Technology; ZJ1, The First Affiliated Hospital of Zhejiang University; ZJ2, The Second Affiliated Hospital of Zhejiang University.
IPM, imipenem; MEM, meropenem; ETP, ertapenem; FOX, cefoxitin; TZP, piperacillin-tazobactam; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; CIP, ciprofloxacin; AMK, amikacin; TGC, tigecycline; POL, polymyxin B.
DE, decreased expression.