TABLE 1.
Time-dependent phosphorylation of l-[3H]Hyd4C in HepG2 cells
| Incubation time (h) | l-Hyd4C concn (μM) | Amt of 5′-phosphorylated metabolite of l-Hyd4C (pmol/106 cells)a |
||
|---|---|---|---|---|
| Monophosphate | Diphosphate | Triphosphate | ||
| 6 | 2 | 0.5 ± 0.1 | 1.3 ± 0.3 | 1.1 ± 0.3 |
| 10 | 4.4 ± 1.2 | 11.5 ± 2.1 | 5.3 ± 1.4 | |
| 12 | 2 | 1.3 ± 0.3 | 4.5 ± 1.2 | 3.1 ± 0.8 |
| 10 | 5.6 ± 1.5 | 22.6 ± 4.1 | 10.4 ± 1.9 | |
| 18 | 2 | 2.3 ± 0.1 | 6.6 ± 1.6 | 3.8 ± 0.8 |
| 10 | 7.3 ± 1.6 | 30.3 ± 4.2 | 15.2 ± 2.7 | |
| 24 | 2 | 4.0 ± 1.0 | 9.8 ± 1.8 | 5.3 ± 1.1 |
| 10 | 11.5 ± 1.8 | 43.5 ± 4.3 | 19.4 ± 2.7 | |
a
The values are means ± standard deviations of results from three independent experiments.