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Melting profiles of DNA gyrase acquired by reading the protein CD signal at 220 nm in 10 mM Tris-HCl (pH 7.5)-20 mM KCl-4 mM MgCl2 in the absence (solid line) and in the presence (dashed line) of 50 μM SD8. The protein concentration was 0.05 μM. Mol. Ell., molar ellipticity.
