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SD8 modulation of the thermal stability of GyrA59 in 10 mM Tris-HCl (pH 7.5)-20 mM KCl-4 mM MgCl2. (A) Melting profiles of GyrA59 acquired by reading the protein CD signal at 220 nm in the absence (solid line) and in the presence (dashed line) of SD8. The protein concentration was 0.2 μM. (B) Variation of the Tm for the thermal transition of GyrA59 as a function of SD8 concentration.
