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Modulation of the melting profiles of GyrB and related subdomains acquired by recording the protein CD signal at 220 nm in 10 mM Tris-HCl (pH 7.5)-20 mM KCl-4 mM MgCl2. (A) Effects of 20 μM ligands on the melting profile of GyrB; (B) effects of increasing SD8 concentrations on the melting profile of GyrB; (C) effects of 20 μM SD8 on the melting profile of GyrB43; (D) melting profile of GyrB47 in the presence or absence of SD8. The protein concentration was 0.2 μM.
