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. 2009 Oct 5;54(1):280–287. doi: 10.1128/AAC.00914-09

FIG. 3.

FIG. 3.

Effects of NRTI on mtDNA content and on the respiratory chain complex activity in differentiating phsPA. (A, C, and E) The mtDNA contents of phsPA differentiated and further incubated till day 30 or 60 in the presence of 6 μM AZT (A), 3 μM d4T (C), or 0.1 μM ddC (E) or vehicle were measured using real-time PCR. (B, D, and F) Respiratory chain complex and citrate synthase activities under the culture conditions described above. Cells were sonicated, and individual substrates were added to the cell lysates, after which levels of substrate conversion by the respiratory chain complexes I and III, II and III, IV, and V and citrate synthase were measured photometrically. All graphs depict data from three independent experiments. Values are means ± SEM. Student's t test and ANOVA with Bonferroni's post hoc analysis were performed for comparison. *, P < 0.05, and **, P < 0.01 for comparison to control data.