FIG. 3.

IB201 protects against S. aureus pneumonia. (A) IB201 was administered to mice via retro-orbital injection 2 h after infection, 12 h after infection, and then every 12 h thereafter. They were challenged with S. aureus Newman via the i.n. route, and mortality was recorded at 24, 48, and 72 hours postinfection (P < 0.039; 15 animals per group) (statistical significance is denoted by an asterisk). (B) CFU recovery from the right lung was determined and demonstrated a significant decrease in bacterial burden in mice that received IB201 (P = 0.008; 15 animals per group). The symbols show the values for individual mice, and the short horizontal lines indicate the mean bacterial load for that group. (C) Histopathology of S. aureus Newman-infected lung tissue from mice that were treated with either PBS or IB201. Scale bars on low-magnification images (left) represent 0.1 cm, while scale bars on high-magnification images (right) represent 20 μm. Experimental results shown in panels A, B, and C are representative of the results in two independent experiments.