FIG. 2.

SDS-PAGE and detection of MccM isolated from E. coli MC4100(pMM75) by gel overlay. Following separation on a 16.5% SDS-tricine polyacrylamide gel, the MccM-containing fraction was stained with Coomassie blue (lane A), subjected to antibacterial assay by gel overlay with the microcin-susceptible strain E. coli RYC1000 (lane B), and submitted to antibacterial assay by gel overlay with MccM-resistant strain E. coli RYC1000(pMcMi) (lane C). The molecular mass ladder (in the first unlabeled lane) was the prestained broad range protein marker (NE Biolabs). Arrows indicate the locations of the band corresponding to MccM.