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. 2009 Nov 2;54(1):288–297. doi: 10.1128/AAC.00744-09

TABLE 1.

Bacterial strains and plasmids used for this study

Strain or plasmid Relevant features Reference or source
E. coli strains
    MC4100 araD139 Δ(argF lac)U169rpsL relAflbBdeoC Laboratory collection
    RYC1000 MC4100 Δrbs-7 recA56 gyrA 12
    W3110 F− IN(rrnD-rrnE)1 Laboratory collection
    KP1344 W3110 (tonB::blaM) 19
    W3110-6 W3110 Δ(exbB-exbD) S. P. Howard, unpublished data
    H1443 MC4100 aroB 15
    H873 H1443 fepA::Tn10 K. Hantke, unpublished data
    H1596 H1443 fiu::Mud X K. Hantke, unpublished data
    H2222 H1443 cir::Mud X K. Hantke, unpublished data
    H1728 H1443 cir fiu::Mud X 14
    H1875 H1443 cir::Mud X fepA::Tn10 14
    H1877 H1443 fepA::Tn10 fiu::Mud X 14
    H1876 H1728 fepA::Tn10 14
    CA46 CIP 105662 Institut Pasteur collection
    CA58 CIP 105663 Institut Pasteur collection
Plasmids
    pJAM229 Derived from pHC79, carrying MccE492 gene cluster, Ampr 30
    pMM21 Carrying MccM, MccI47, and MccH47 gene clusters; Ampr F. Moreno, unpublished data
    pMM75 Carrying MccM, MccI47, and MccH47 gene clusters; mchB inactivation; Ampr Kanr F. Moreno, unpublished data
    pMM1.15 Carrying MccM, MccI47, and MccH47 gene clusters; mcmA inactivation; Ampr, Kanr F. Moreno, unpublished data
    pMcMi Carrying mcmI from MccM gene cluster, Ampr F. Moreno, unpublished data
    pMcHi Carrying mchI from MccH47 gene cluster, Cmr F. Moreno, unpublished data