Confirmation of B virus isolate genotype by RFLP. Viral DNA was digested with the SalI restriction enzyme and was separated on a 0.8% agarose gel. MR, rhesus macaque isolate; E2490, prototype laboratory strain; A, human isolate; MJ, Japanese macaque isolate; MC, cynomolgous macaque isolate; MP, pigtail macaque isolate. Size markers are indicated in kilobase pairs and reflect the positions of BstEII-digested lambda DNA fragments.