TABLE 3.
MICs and the embCAB mutations of M. tuberculosis strain 210 and its in vitro-selected clones
| EMB exposure concn (μg/ml) | M. tuberculosis strain | EMB MIC (μg/ml) by the following method: |
embCAB mutation | |
|---|---|---|---|---|
| 7H10 agar proportion | Bactec 460TB | |||
| NAa | 210 | 2 | 2 | Wild type |
| 8 | 8C1 | 6 | 2 | Wild type |
| 8C2 | 8 | 4 | embB wild type | |
| 8C3 | NDb | 4 | ND | |
| 8C5 | 8 | 4 | embC13 CGG-CTG (Arg-Leu)c | |
| 8C6 | 4 | 2 | Wild type | |
| 8C7 | 4 | 2 | embB445 CAG-CGG (Gln-Arg)c | |
| 16 | 16C6 | 8 | 8 | embB320 TTC-TTG (Phe-Leu)c |
| 16C7 | ND | 8 | embB445 CAG-CGG (Gln-Arg)c | |
| 16C9 | ND | 12 | embB497 CAG-CGG (Gln-Arg)d | |
| 16C10 | 6 | 2 | Wild type | |
| 16C11 | 8 | 5 | embB397 CCG-TCG (Pro-Ser)2 | |
| 16C12 | 8 | 3 | Wild type | |
| 16C14 | 16 | 8 | embB445 CAG-CGG (Gln-Arg)c | |
| 16C15 | ND | 12 | embB497 CAG-CGG (Gln-Arg)d | |
| 16C16 | 8 | 3 | embB1024 GAC-AAC (Asp-Asn)c | |
| 32 | 32C1 | 6 | 2 | embB445 CAG-CGG (Gln-Arg)c |
| 32C2 | ND | 12 | embB497 CAG-CGG (Gln-Arg)d | |
| 32C3 | ND | 12 | embB497 CAG-CGG (Gln-Arg)d | |
| 32C4 | ND | 4 | embB445 CAG-CGG (Gln-Arg)c | |
| 32C5 | 8 | 8 | embB324 GGC-CGC (Gly-Arg)c | |
a
NA, not applicable.
b
ND, not done.
c
Mutations detected by embB gene sequencing and/or embCAB operon sequencing.
d
Mutations detected by molecular beacon assays.