FIG. 1.

Time- and dose-dependent reduction of viral parameters in genotype 1b (Con1) replicon cells induced by compound 6. Huh-luc/neo-ET cells were incubated with serially diluted compound 6 for 24, 48, or 72 h. Results are expressed as percentages of the level for mock-treated controls. (A) Reduction of reporter luciferase activity, which indirectly reflects the replication level of HCV replicons. (B) Reduction of HCV RNA level normalized against the mRNA level of human β-actin. (C) Reduction of the NS5A protein level as in one experiment. (D) NS5A protein level quantitated and normalized against the protein level of α-tubulin (means ± standard deviations [SD] of results from three independent experiments).