TABLE 5.

Gene expression analyses of select transcriptional regulators of S. Enteritidis^a

Strain	Change in gene expression (P)b
	acrB		soxS		marA		ramA		rob
	RT-PCR	Array	RT-PCR	Array	RT-PCR	Array	RT-PCR	Array	RT-PCR	Array
104-cip	6.1 ± 1.5	2.22 (2.4E−11)	26.1 ± 4.0	1.19 (3.5E−05)	8.9 ± 0.6	0.15 (0.07)	1.2 ± 0.2	0.16 (0.5)	−4.6 ± 0.2	−0.8 (3.2E−06)
5408-cip	5.4 ± 1.6	1.53 (1.8E−06)	−3.4 ± 0.5	−0.15 (0.25)	1.3 ± 0.2	0.17 (0.03)	33.7 ± 4.0	0.9 (1.1E−03)	−2.3 ± 1.0	−0.34 (2.2E−03)

^aRNA was extracted from cultures growing at log phase in LB. RT-PCR data represent means ± SD of results from 3 independent total RNA extractions. Changes in gene expression are relative to the levels for the respective parental strains.

^bArray data were gathered from six biological replicates. Analysis was done using WebArray (46), employing the print-tip Loess and scale methods for within- and between-array normalizations, respectively. The cutoff values for genes downregulated and upregulated in the ciprofloxacin-resistant mutants (log₂ ciprofloxacin-resistant-mutant/wild-type ratio values) were <1 (P < 0.01) and >1.0 (P < 0.01), respectively. Log₂ ciprofloxacin-resistant-mutant/wild-type ratio values with P values greater than 0.01 are marked in italics to indicate lower confidence levels.