TABLE 2.
Selected genes that displayed altered expression in the presence of tigecycline, as determined by microarray analysis and real-time RT-PCR
| Functional group | Gene | Protein | Fold change in expression measured by: |
|
|---|---|---|---|---|
| Microarray analysis | Real-time RT-PCR | |||
| Protein synthesis, ribosomes | rpsH | 30S ribosomal protein S8 | 14.86 | |
| rplN | 50S ribosomal protein L14 | 13.76 | ||
| rplB | 50S ribosomal protein L2 | 13.38 | ||
| rplD | 50S ribosomal protein L4 | 13.06 | ||
| rplJ | 50S ribosomal protein L10 | 12.75 | ||
| rplC | 50S ribosomal protein L3 | 12.06 | ||
| rplK | 50S ribosomal protein L11 | 11.03 | ||
| rpsE | 30S ribosomal protein S5 | 11.01 | ||
| rpmI | 50S ribosomal protein L35 | 10.51 | ||
| Protein synthesis, translation and modification | fus | Elongation factor G | 10.51 | |
| tuf | Elongation factor Tu | 7.41 | ||
| tsf | Elongation factor Ts | 3.23 | ||
| infC | Initiation factor C | 6.29 | ||
| infA | Initiation factor A | 4.77 | ||
| infB | Initiation factor B | 2.35 | ||
| rho | Transcription termination factor | 2.15 | ||
| Cell envelope, surface proteins and antigens | capB | Capsular polysaccharide synthesis enzyme | −2.08 | |
| cap8H | Capsular polysaccharide synthesis enzyme | −2.18 | ||
| capD | Capsular polysaccharide synthesis enzyme | −2.19 | ||
| capG | Capsular polysaccharide synthesis enzyme | −2.30 | −1.01 | |
| capF | Capsular polysaccharide synthesis enzyme | −2.66 | ||
| capC | Capsular polysaccharide synthesis enzyme | −3.14 | −1.75 | |
| icaC | Intercellular adhesion protein C | −2.06 | −2.23 | |
| Cell envelope, surface anchored membrane exported lipoproteins | fnbA | Fibronectin-binding protein | 2.30 | |
| fnbB | Fibronectin-binding protein | 5.34 | 3.03 | |
| clfB | Fibrinogen- and keratin 10-binding surface anchored protein | 4 | 7.5 | |
| sasF | Putative surface anchored protein | −2.30 | −1.32 | |
| cna | Collagen adhesion precursor | 2.10 | 3.42 | |
| sdrD | Fibrinogen-binding protein | −2.46 | ||
| Cell envelope, exported lipoproteins | sstD | Lipoprotein | 3.27 | |
| sbi | IgG-binding protein | 2.39 | ||
| ssaA | Secretory antigen precursor | 2.84 | 2.04 | |
| isaA | Immunodominant antigen A | 5.08 | 7.68 | |
| Cell envelope-peptidoglycan | dltA | d-Alanine-d-alanyl carrier protein ligase | 2.63 | |
| dltB | Putative activated d-alanine transport protein | 2.02 | ||
| dltC | d-Alanyl carrier protein | 2.29 | ||
| pbp2 | Penicillin-binding protein 2 | 3.91 | ||
| DNA, replication and repair | nusG | Transcription antitermination protein | 4.34 | |
| rpoC | DNA-directed RNA polymerase protein | 3.28 | ||
| topA | DNA topoisomerase I | 2.26 | ||
| recU | Putative recombination protein | 4.38 | ||
| dnaG | DNA primase | 2.19 | ||
| polA | DNA polymerase I | −2.11 | ||
| Global regulatory functions | mecI | Methicillin resistance regulatory protein MecI | −2.39 | |
| clpC | Putative stress response-related Clp ATPase | −2.44 | −1.14 | |
| sarA | Staphylococcal accessory regulator A | 2.37 | ||
| saeR | Response regulator protein | 2.02 | ||
| gapR | Glycolytic operon regulator | 9.12 | ||
| dinR | DNA damage-inducible repressor | −3.31 | ||
| tcaR | MarR family regulatory protein | 2.79 | ||
| icaR | ica operon transcriptional regulator | −3.48 | ||
| Cellular processes, transport | mnhF | Na+/H+ antiporter subunit | 3.27 | |
| mnhE | Na+/H+ antiporter subunit | 3.15 | ||
| mnhG | Na+/H+ antiporter subunit | 2.85 | 5.16 | |
| mnhD | Na+/H+ antiporter subunit | 1.93 | ||
| Adaptation to atypical conditions | sodA | Superoxide dismutase | −2.59 | |
| clpB | Putative ATPase subunit of an ATP-dependent protease | −2.49 | ||
| asp23 | Alkaline shock protein 23 | −8.61 | ||
| cspB | Cold shock protein | 5.59 | ||
| Toxin production | tst | TSST-1 | −2.39 | −1.51 |