TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | References or source |
|---|---|---|
| S. pneumoniae strains | ||
| D39 | Serotype 2 strain, cps2 | 1, 39; source was the group of P. W. Hermans |
| D39nisRK | D39 ΔbgaA::nisRK; Tmpr | 29 |
| Δ385-387 | D39 ΔSP0385-0387; Sptr | This work |
| Δ785-787 | D39 ΔSP0785-0787; Eryr | This work |
| Δ912-913 | D39 ΔSP0912-0913; Eryr | This work |
| Δ1714-1715 | D39 ΔSP1714-1715; Eryr | This work |
| Δ1715 | D39 ΔSP1715; Eryr | This work |
| Δblp strain | D39 ΔSPD0473-0476; Eryr | This work |
| OV912 | D39 nisRK/pNZ912; Cmr | This work |
| OV1715 | D39 nisRK/pNZ1715; Cmr | This work |
| CO912 | OV912 Δ912-913 | This work |
| CO1715 | OV1715 Δ1715 | This work |
| CO1716 | OV1715 Δ1714-1715 | This work |
| DM39 | Δ385-387 Δ912-913 | This work |
| DM19 | Δ912-913 Δ1714-1715 | This work |
| PR385 | D39 ΔbgaA::PSP0385-lacZ; Tetr | This work |
| PR785 | D39 ΔbgaA::PSP0785-lacZ: Tetr | This work |
| PR912 | D39 ΔbgaA::PSP0912-lacZ; Tetr | This work |
| PR1714 | D39 ΔbgaA::PSP1714-lacZ; Tetr | This work |
| PR785Δ1714 | PR785/Δ1714-1715 | This work |
| PR912Δ1714 | PR912/Δ1714-1715 | This work |
| PR1714Δ1714 | PR1714/Δ1714-1715 | This work |
| E. coli EC1000 | MC1000 derivative carrying a single copy of the pWV01 repA gene in glgB; Kmr | 40 |
| L. lactis NZ9000 | MG1363 ΔpepN::nisRK | 35 |
| Plasmids | ||
| pPP2 | Promoterless lacZ, for replacement of bgaA (spr0565) with promoter-lacZ fusions, derivative of pPP1; Ampr Tetr | 19 |
| pNZ8048 | Nisin-inducible PnisA; Cmr | 13 |
| pPA1 | pPP2 PSP0385-lacZ | This work |
| pPA2 | pPP2 PSP0785-lacZ | This work |
| pPA3 | pPP2 PSP0912-lacZ | This work |
| pPA4 | pPP2 PSP1714-lacZ | This work |
| pNZ912 | pNZ8048 carrying SP0912-0913 downstream of PnisA | This work |
| pNZ1715 | pNZ8048 carrying SP1715 downstream of PnisA | This work |
a
Eryr, erythromycin resistance; Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Sptr, spectinomycin resistance; Tmpr, trimethoprim resistance.