TABLE 2.

Oligonucleotide primers used in this study

Oligonucleotide primer	Nucleotide sequence (5′ to 3′)a	Restriction site
KN-sp912-913-for-1	GGAAGCCAGCCACAGGCTGTA
KN-sp912-913-rev-2	GAGATCTAATCGATGCATGCGTGTCATGAGAATCTCCTTTC
KN-sp912-913-for-3	AGTTATCGGCATAATCGTTACTTCCTCATCGCCTATGTGCTG
KN-sp912-913-rev-4	CGTAGATGGTTACCTAAGGGAACC
KN-sp785-787-for-1	TGACAGGGACTTTGTGAGTGTG
KN-sp785-787-rev-2	GAGATCTAATCGATGCATGCCCCTCCAGCAAACAATACA
KN-sp785-787-for-3	AGTTATCGGCATAATCGTCAACAAGATGGACACTCGTCT
KN-sp785-787-rev-4	GGAAGACTGTTCCATTCCAGAA
KN-sp385-387-for-1	GTGCCACCATAGCAGATCTACAA
KN-sp385-387-rev-2	CCTCCTCACTATTTTGATTAGTATGAGAGCAATAATGACATAGGC
KN-sp385-387-for-3	TGGGAAATATTCATTCTAATTGGCCATTTGGTGGGGCAAGAGGAG
KN-sp385-387-rev-4	TCACGCTAGAGGTACTTGCTTGC
KN-sp1714-1715-for-1	TCAGTGCCTCCTGACCGATAATCGGG
KN-sp1714-1715-rev-2	GAGATCTAATCGATGCATGCTTGGTCTCCTTTCTCTTACCC
KN-sp1714-1715-for-3	AGTTATCGGCATAATCGTTACTCGGAACCTACTACATCTTGA
KN-sp1714-1715-rev-4	GTGACAGCTCTAGGTGCAGCT
KN-sp1715-for-1	CTTGACACAGGACGTTTCTGGGCT
KN-sp1715-rev-2	GAGATCTAATCGATGCATGCCATTTTCAAATGCTAGTAATGACAT
KN-sp1715-for-3	AGTTATCGGCATAATCGTTACTCGGAACCTACTACATCTTGA
KN-sp1715-rev-4	GTGACAGCTCTAGGTGCAGCT
KN-blp-for-1	CTCATCCAAGATTCCTTGGAGAT
KN-blp-rev-2	GAGATCTAATCGATGCATGCAGCCACCTCTATTTCAAGCCACC
KN-blp-for-3	AGTTATCGGCATAATCGTCGAGACAAGTATGGAAAGAG
KN-blp-rev-4	CAAAGCGTTCTACTGTACCAGACAT
Oversp912-913;fv	CATGCCATGGCACTTTTAGATGTAAAACACG	NcoI
Oversp912-913;rev	GCTCTAGAATACCTCGATTTTGAAGTCGAGG	XbaI
Oversp1715;fv	CATGCCATGGCATTACTAGCATTTGAAAATG	NcoI
Oversp1715;rev	GCTCTAGATGAGTATGTTACATATCTAGG	XbaI
Psp385-387-fv	CGGAATTCGTGCCACCATAGCAGATCTACA	EcoRI
Psp385-387-rev	GCTCTAGACTCATAGGTTCATCCTCTCCCT	XbaI
Psp785-787-fv	CGGAATTCTCCGCTACCTCCACCGATAGCAAT	EcoRI
Psp785-787-rev	GCTCTAGACTTCATAATGAAACTCCTTTTC	XbaI
Psp912-913-fv	CGGAATTCTGGATGCTGATAACAACTGATAAC	EcoRI
Psp912-913-rev	GCTCTAGAGTGTCATGAGAATCTCCTTTCT	XbaI
Psp1714-1715-fv	CGGAATTCCTACGAATGGTGTTCCCTTCT	EcoRI
Psp1714-1715-rev	GCTCTAGATGTCAAATGTCCAGGACATC	XbaI

^aRestriction enzyme sites are underlined.