TABLE 2.

Oligonucleotides used in PCR and qRT-PCR

Gene	Primer	Sequence (5′→3′)	Positiona	Size (bp)
rpoB	rpoB Rev	ATGCCGCCTGAAAAAGTAAC	1960-1979	154
	rpoB For	TCCGCACGTAAAGTAGGAAC	2114-2095
adeA	adeA For	ATCGCTAACAAAGGCTTGAA	1024-1043	159
	adeA Rev	CGCCCCCTCAGCTATAGAA	1183-1163
adeB	adeB For	CTTGCATTTACGTGTGGTGT	2907-2926	168
	adeB Rev	GCTTTTCTACTGCACCCAAA	3075-3056
adeC	adeC For	TACACATGCGCATATTGGTG	1157-1176	117
	adeC Rev	CGTAAAATAACTATCCACTCC	1274-1244
adeI	adeI For	CAAATGCAAATGTAGATCTTGG	511-532	210
	adeI rev	AAACTGCCTTTACTTAGTTG	702-721
adeJ	adeJ For	GGTCATTAATATCTTTGGC	1142-1160	221
	adeJ Rev	GGTACGAATACCGCTGTCA	1363-1345
adeK	adeK For	TTGATAGTTACTTGACTGTTC	1264-1285	162
	adeK Rev	GGTTGGTGAACCACTGTATC	1407-1426
ampC	ampC For	CAGTAATTCAGAACAGATTGTG	927-947	183
	ampC Rev	GCGCTCTTCATTTGGAATAC	1110-1091
adeS	adeS For	TATGAAAAGTAAGTTAGGAAT	1-19	1,072
	adeS Rev	TTAGTTATTCATAGAAATTTT	1073-1053
adeR	adeR For	AATGCTTAATACACTGACTTTAGCCGGTGG	−265-−236	1,429
	adeR Rev	TCTAGCAGAGAGGTCGC	1101-1084
ISAba1	ISAba1ab	ATGCAGCGCTTCTTTGCAGG	462-471	379
	ISAba1bb	AATGATTGGTGACAATGAAG	822-841
ISAba4	ISAba4ac	ATTTGAACCCATCTATTGGC	140-159	611
	ISAba4bc	ACTCTCATATTTTTTCTTGG	751-732
blaOXA-23	oxa23+	ATGAATAAATATTTTACTTG	1-20	821
	oxa23−	TTAAATAATATTCAGCTGTT	822-803
tniA	tniA For	CAATGTGGTTGTCGGTTATGA	54-74	162
	tniA Rev	ATTCAAACTCTCAGTTAGCGT	216-196
ATPase	ATPase3′	AATGGCGGTACATCAATATGT	1215-1195	492
	ATPase5′	GAAACTCTTGAAGTTGCAAGT	723-743

^aCoordinates refer to the first base of each gene.

^bFrom reference 20.

^cFrom reference 8.