TABLE 4.
Microbicidal activity of modified amino acid sequence 20-31 (CFQWQRNMRKVR)a
| Codeb | MMC99 μg/ml |
||
|---|---|---|---|
| E. coli | S. aureus | C. albicans | |
| Positively charged amino acids | |||
| Orn or K substitution | |||
| CFQWOrnRNMRKVR | 25 | 50 | 25 |
| CFQWKRNMRKVR | 25 | 25 | 25 |
| CFQWQROrnMRKVR | 25 | 50 | 25 |
| Hydrophobic amino acids | |||
| Nle substitution | |||
| CFQWNleRNMRKVR | 50 | 50 | 25 |
| CFQWQRNleMRKVR | 50 | 50 | 50 |
| Two or more substitutions | |||
| CFQWKRAMRKVR (HLopt2) | 12 | 12 | 12 |
| CFAWKRNMRKVR | 50 | 25 | 25 |
| CFAWQRAMRKVR | 25 | 50 | 25 |
| CFALKKAMKKVR | 25 | 50 | 25 |
| Native sequence | |||
| 20CFQWQRNMRKVR31 | 100 | >100 | 50 |
a
The peptides were incubated with E. coli O6K5, S. aureus 1800 and C. albicans in BP. The peptides were diluted in 2-fold steps, starting at 100 μg/ml. All samples were run in duplicate. Orn, ornithine, an amino acid not coded for by DNA and a metabolic intermediate; Nle, norleucine, a nonnatural synthetic amino acid.
b
Substitutions are shown in bold type.