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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Neurobiol Dis. 2009 Aug 20;36(2):374–383. doi: 10.1016/j.nbd.2009.08.003

Figure 1. Rab11 but not Rab5 activation is inhibited in HD brains.

Figure 1

A and B, 200μg of striatal total membranes prepared from freshly sacrificed WT and homozygous HD mice (see Methods) were used as the source of GEFs for catalyzing [3H]GDP release from [3H]GDP-GST-Rab11 (A) or [3H]GDP-6×His-Rab5 (B) for indicated times. Data were represented as the percentage of [3H]GDP released from GST-Rab11 or 6×His-Rab5 after incubation with striatal total membranes (n=3 for each time point in both A and B, Mean±SD, 2-tailed Student t-test: * p<0.01). C, Rab11-enriched membrane fractions (fraction-7 for HD and fractions-6 and 7 for WT) were prepared as described in Methods. 50μg of HD and WT mouse brain Rab11-enriched membranes were used to catalyze [3H]GDP release from GST-Rab11 for indicated times. Data are represented as the percentage of [3H]GDP released from GST-Rab11 after incubation with Rab11-enriched membrane fractions (n=3 for each time point, Mean±SD, 2-tailed Student t-test: * p<0.05).