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. Author manuscript; available in PMC: 2009 Dec 28.
Published in final edited form as: Gene Ther. 2009 Feb 19;16(5):581–588. doi: 10.1038/gt.2009.16

Figure 2.

Figure 2

Summary of the somatic cell nuclear transfer (SCNT) approach used to produce CFTR gene-targeted ferrets. Primary fetal ferret fibroblasts are infected with rAAV-targeting virus and subsequently cloned by limiting dilution under G418 selection. Following replica plating and PCR screening in 96-well plates, cells are minimally expanded and then used for SCNT. Embryo fibroblasts (21 days) are then derived under G418 selection and screened by Southern blotting for the CFTR gene-targeting event. Rejuvenated fibroblasts containing the CFTR-targeted allele are then used for a second round of SCNT to clone live ferrets.