Figure 4.

Irradiated defects regenerated with AdBMP-2 lyophilized in gelatin scaffolds. To test the stability of viruses in the gelatin scaffolds, we stored pre-made AdBMP-2-lyophilized scaffolds (1M-PreOP) at -80°C for 1 mo prior to surgery. (A) The 3-D images were reconstructed to illustrate the top and sagittal section views. The bone regeneration was compared with that in the radiated group before storage (Pre-OP) by µ-CT analyses: (B) BVF, (C) BAF, and (D) BMD. The data were expressed as mean ± standard deviation, and the statistical differences between groups were analyzed by Student's t test (*p < 0.05; **p < 0.01). The dotted line represents the surgical margin of the cranial defect. N = 5.