FIG. 1.

Molecular phylogenetic analysis of the endosymbionts of Curculio weevils on the basis of 16S rRNA gene sequences. A Bayesian tree inferred from 1,180 unambiguously aligned nucleotide sites is shown, whereas a maximum likelihood analysis gave substantially the same topology (data not shown). Posterior probabilities for the Bayesian analysis and bootstrap values (>50%) for the maximum likelihood analysis are shown above and below the nodes, respectively. Sequence accession numbers and AT contents of the nucleotide sequences are in brackets and parentheses, respectively. As for insect endosymbionts, name of the host insect is also indicated in parentheses. P-symbiont, primary symbiont; S-symbiont, secondary symbiont.