FIG. 2.

(A) An adult insect of C. sikkimensis. (B to F) Localization of the primary symbiont in dissected tissues of C. sikkimensis. Red and green signals indicate 16S rRNA of the primary symbiont and nuclear DNA of the insect cells, respectively. (B) Location of the bacteriome visualized on a dissected intestine of a final instar larva; (C and D) enlarged images of the bacteriome, wherein many bacteriocytes loosely aggregate and surround the midgut tube; (E) an enlarged image of the bacteriocytes, whose cytoplasm is filled with filamentous bacterial cells; (F) the apex of an ovary dissected from an adult female, wherein the symbiont signals are seen; (G) freshly isolated symbiont cells stained with SYTOX green.