FIG. 1.

Screening of C. albiflorum leaf and bark extracts for anti-QS activity using C. violaceum CV026 (A) and P. aeruginosa PAO1 (B). (A) Growth and violacein production by C. violaceum CV026 when HHL is supplied at a final concentration of 3 μM and in the presence of C. albiflorum extracts. (B) Growth and pyocyanin production by P. aeruginosa PAO1 when C. albiflorum extracts are added to the growth medium. DMSO-supplied cultures were used as controls. Violacein and pyocyanin were extracted as described in Materials and Methods and quantified by spectrophotometry (575 nm for violacein and 380 nm for pyocyanin). Bacteria growth was assessed at 600 nm. All graphs show the OD values associated with each parameter measured, and each test was performed four times. ***, Data that are statistically different (P ≤ 0.01).