FIG. 3.

Modeled structure of full-length XYN10A xylanase. The model was created by SWISS-MODEL using 1v6w as a template, and the figure was made using PyMOL (11). The residue Asn301 is the C terminus of the expressed catalytic core. The residue Ala1 (A1) shows the position of the N terminus. The glycosylation site Asn26 and the positively charged residues (His12, Arg14, Arg36, Arg219, Arg252, and Lys289) in the range of the 6×His tag are shown as one-letter codes. The sequence positions corresponding to the 6×His tag (positions 302 to 307 in full-length XYN10A) are shown in magenta, although the conformation of the 6×His tag is not known. The active site is located on the other side of the barrel.