FIG. 4.

Listeria monocytogenes prfA (A) and inlA (B) transcription measured by Northern blotting using RNA isolated from L. monocytogenes EGD grown in BHI-AC (0.2% charcoal) for 15 min (lanes A, B, and C), 30 min (lanes D, E, and F), 60 min (lanes G, H, and I), or 180 min (lanes J, K, and L) with water (control) (lanes A, D, G, and J) or 0.125% (lanes B, E, H, and K) or 0.250% (lanes C, F, I, and L) Incimaxx DES. The arrows show the plcA-prfA (2.1-kb), prfA (0.8- and 0.9-kb), inlAB (5-kb), and inlA (2.9-kb) transcripts. The results are representative of two independent experiments.