FIG. 2.

Fluorescence microscopy of cells containing GFP-fused ABC transporters grown in the absence or presence of 0.2 g liter⁻¹ iso-α-acid-enriched hop extract. Cells were grown in shake flasks containing mineral medium supplemented with glucose, vitamins, and uracil in the presence or absence of iso-α-acid-enriched hop extract. Zeiss filter set FS10 (excitation bandpass, 450 to 490 nm; emission bandpass, 515 to 565 nm) was used for GFP studies, and images were taken with a Zeiss Axiocam MRc (exposure time, 547 ms). The arrow indicates the localization of Pdr5p in the plasma membrane of yeast cells grown in the presence of 200 ppm hops. Panels: A, isogenic reference CEN.PK113-5D; B, IMN008 (Pdr5::Gfp); C, IMN009 (Pdr15::Gfp); D, IMN011 (Tpo1::Gfp); E, IMN010 (Snq2::Gfp). FITC, fluorescein isothiocyanate; PC, phase-contrast.