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Phenotypic analysis of aft1Δ and zap1Δ mutants. The strains (BY4741 and the aft1Δ and zap1Δ mutants) were spotted at 105 cells ml−1 (and three 10-fold serial dilutions) on synthetic agar medium (SM; containing 10 μM Fe and 15 μM Zn) supplemented with either 100 μM iron or 750 μM zinc in the presence of 0.2 or 1 g liter−1 hop iso-α-acids.
