TABLE 1.
Strains used in this study
| Strain | Genotypea | Source or reference |
|---|---|---|
| Open Biosystems | MATahis3Δ1leu2Δ0met15Δ0ura3Δ0gene::loxP-kanMX-loxP | Open Biosystems |
| Knockout collection | ||
| CEN.PK113-7D | MATaMAL2-8cSUC2 | P. Kötterb |
| CEN.PK113-5D | MATaMAL2-8cSUC2ura3 | P. Kötter |
| IMK123 | MATaMAL2-8cSUC2ura3pdr1::loxP-kanMX-loxP | This study |
| IMK124 | MATaMAL2-8cSUC2ura3pdr3::loxP-kanMX-loxP | This study |
| IMK130 | MATaMAL2-8cSUC2ura3snq2::loxP-kanMX-loxP | This study |
| IMK131 | MATaMAL2-8cSUC2ura3pdr5::loxP-kanMX-loxP | This study |
| IMK132 | MATaMAL2-8cSUC2ura3pdr15::loxP-kanMX-loxP | This study |
| IMK133 | MATaMAL2-8cSUC2ura3pdr3::loxPpdr1::loxP-kanMX-loxP | This study |
| IMK150 | MATaMAL2-8cSUC2ura3pdr5::loxPpdr15::loxPsnq2::loxP-kanMX-loxP | This study |
| IMK241 | MATaMAL2-8cSUC2ura3pdr3::loxPpdr1::loxP-kanMX-loxPyrm1::hphNT1 | This study |
| IMK246 | MATaMAL2-8cSUC2ura3pdr3::loxPpdr1::loxP-kanMX-loxPyrm1::hphNT1yrr1::natNT2 | This study |
| IMK159 | MATaMAL2-8cSUC2ura3pdr3::loxPpdr1::loxP-kanMX-loxP p426GPD(URA3) | This study |
| IMZ110 | MATaMAL2-8cSUC2ura3pdr3::loxPpdr1::loxP-kanMX-loxPyrm1::hphNT1yrr1::natNT2 p426GPD (URA3) | This study |
| IMN008 | MATaMAL2-8cSUC2ura3pdr5::yEGFP-hph | This study |
| IMN009 | MATaMAL2-8cSUC2ura3pdr15::yEGFP-hph | This study |
| IMN010 | MATaMAL2-8cSUC2ura3snq2::yEGFP-hph | This study |
| IMN011 | MATaMAL2-8cSUC2ura3tpo1::yEGFP-hph | This study |
a
Knocked-out genes are in boldface. yEGFP, yeast enhanced GFP; gene, any of the 4,786 open reading frames that comprise the MATa yeast knockout collection (Open Biosystems, Huntsville, AL).
b
Institut für Mikrobiologie der J. W. Goethe Universität.