TABLE 5.
Hop-sensitive mutants identified by screening the Open Biosystems deletion collection
| Cellular function and gene(s) | Description |
|---|---|
| Vacuolar acidification | |
| VMA6, VMA9 | V0 domain subunits |
| VMA1, VMA5, VMA8, VMA10, VMA13 | V1 domain subunits |
| VPH2, VMA21, VMA22 | V-ATPase assembly factors |
| Protein trafficking | |
| VPS16 | Vacuolar protein sorting |
| CHC1a | Clathrin coat required for internalization step of endocytosis |
| VPS52a, VPS53a, VPS54a | Vacuolar protein sorting |
| Metal homeostasis | |
| AFT1 | Iron-responsive transcription factor |
| ZAP1a | Zinc-activated protein |
| Other | |
| OCH1 | α-1,6-Mannosyltransferase, initiation of mannose outer chain elongation |
| FUN32 | Regulatory A subunit of protein serine-threonine phosphatase 2A, activated by ceramide |
| RNR4a | Ribonucleotide reductase 4, required for normal levels of translesion synthesis DNA repair |
a
The corresponding mutant was only sensitive to 1 g liter−1 hop iso-α-acids. All other mutants were sensitive to 0.2 g liter−1 hop iso-α-acids.