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Intracellular (triangles) and extracellular (squares) concentrations of galactarate produced by H. jecorina QM6a Δgar1 udh from d-galacturonate (circles) in pH-regulated bioreactors at pH 4.0 (solid symbols) and pH 6.5 (open symbols). Cultures were inoculated with 1.8 g of biomass liter−1 and maintained at 30°C and 500 rpm with 1 vvm aeration. In addition to d-galacturonate, the medium contained 4 g of glucose liter−1 and mineral salts as described in Materials and Methods.
