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. 2009 Nov 6;76(1):169–175. doi: 10.1128/AEM.02273-09

TABLE 1.

Primers used for PCR

Primer Sequence
gar1 5f GTAACGCCAGGGTTTTCCCAGTCACGACGAAGCTTATATCCACCGTGTCCCAG
gar1 5r ATCCACTTAACGTTACTGAAATCTCCAACGACGCAGTTGTTTGAGCAAC
gar1 3f CTCCTTCAATATCATCTTCTGTCTCCGACTTGCATTGGTCAGAGCGGTA
gar1 3r GCGGATAACAATTTCACACAGGAAACAGCAAGCTTAAGCAGTGGATGACTTGCTG
hphF GTCGGAGACAGAAGATGATATTGAAGGAGC
hphR GTTGGAGATTTCAGTAACGTTAAGTGGAT
pyrG-5-F TATAGAATTCTGCGGGCATGATGTTTCAAC
pyrG-5-R TATACCCGGGAAATCATCGCGAGCCCCT
pyrG-3-F_n TATACCCGGGATGTCGAGCACGGGTAGTCA
pyrG-3-R_n TATAGAATTCATGCAAGCGGCAGAGTACTTT
gaaA-5-F TATACTCGAGTGAATTGCACTCTTCGTACCG
gaaA-5-R TATACCATGGTGTGATTGCTGTGGTGTAAAT
gaaA-3-F TATACCATGGCCGTTTATGATTCTGGTCCATC
gaaA-3-R TATAGAATTCTCGAGTTAATTCCCTTAGCG
pyrG-del-F_n TATACCCGGGTGATTGAGGTGATTGGCGAT
pyrG-del-R_n TATACCCGGGTTATCACGCGACGGACAT