FIG. 6.

In vitro simultaneous bioluminescence monitoring of various bacterial mixtures of green light- and red light-emitting E. coli 55989-Str^r. (A) Spectral emissions of the green light-emitting (Val₂₄₁Ile/Gly₂₄₆Ala/Phe₂₅₀Ser) and red light-emitting (Ser₂₈₄Thr) luciferase mutants. (B) Suspensions of ca. 10⁸ CFU/ml of mixed green light- and red light-emitting bacteria at ratios of 0:100, 20:80, 40:60, 60:40, 80:20, and 100:0 were analyzed. Similar ratios of green light- or red light-emitting bacteria with nonbioluminescent E. coli 55989-Str^r were analyzed. The enzymatic reaction was initiated by adding chromaGlo reagent just before imaging. Mean values are plotted for each dilution, and standard errors are indicated by the bars.