TABLE 1.
Strains of bifidobacteria, fermentation on LNB, and detection of the GLNBP gene lnpA by PCR
| Speciesa | Strain(s) | Origin | PCR detection of lnpAb | Growth on LNBb | Growth pattern onc: |
|||
|---|---|---|---|---|---|---|---|---|
| LNB | Lactulose | Raffinose | Glucose | |||||
| B. longum subsp. longum | IV-51 (same as ATCC 15707T) | Feces of adult | + | 5 | 4 | 5 | 5 | |
| ATCC 15708 | Feces of infant | + | 2 | 2 | 4 | 4 | ||
| ATCC BAA-999 (BB536) | Feces of infant | + | 5 | 5 | 0 | 5 | ||
| MCC strains | Feces of adult or infant | 3/3 | 3/3 | 2-5 | 5 | 4-5 | 5 | |
| B. longum subsp. infantis | IV-8 (same as ATCC 15697T) | Feces of infant | + | 5 | 5 | 5 | 5 | |
| MCC strains | Feces of adult, infant, or from commercial products | 13/13 | 13/13 | 2-5 | 1-5 | 0-5 | 2-5 | |
| B. longum subsp. longum/infantis | MCC strains | Feces of adult, infant, or from commercial products | 7/7 | 7/7 | 2-5 | 5 | 5 | 2-5 |
| B. breve | IV-14 (same as ATCC 15700T) | Feces of infant | + | 5 | 5 | 5 | 5 | |
| MCC strains | Feces of infant or from yogurt | 8/8 | 8/8 | 2-5 | 3-5 | 2-5 | 1-5 | |
| B. bifidum | IV-127 (same as ATCC 29521T) | Feces of infant | + | 1 | 3 | 0 | 3 | |
| ATCC 15696 | Intestine of infant | + | 5 | 5 | 0 | 4 | ||
| MCC strains | Feces of infant | 4/4 | 4/4 | 5 | 5 | 0 | 3-5 | |
| B. adolescentis | ATCC 15706 | Feces of adult | − | 0 | 1 | 2 | 2 | |
| ATCC 15705 | Feces of adult | − | 0 | 2 | 0 | 2 | ||
| ATCC 15704 | Feces of adult | − | 0 | 1 | 0 | 1 | ||
| MCC strains | Feces of adult or infant | 0/48 | 0/48 | 0 | 0-3 | 0-4 | 0-4 | |
| B. catenulatum | ATCC 27675 | Feces of human | − | 0 | 2 | 1 | 2 | |
| JCM7130 | Sewage | − | 0 | 4 | 4 | 4 | ||
| MCC strains | Feces of adult | 0/11 | 0/11 | 0 | 1-5 | 1-5 | 2-5 | |
| B. pseudocatenulatum | IV-152 (same as ATCC 27919T) | Feces of infant | − | 4 | 5 | 5 | 5 | |
| ATCC27676 | Sewage | − | 4 | 5 | 5 | 5 | ||
| ATCC27677 | Sewage | − | 4 | 4 | 5 | 4 | ||
| IV-40 (same as JCM7040) | Feces of adult | − | 5 | 5 | 5 | 5 | ||
| MCC strains | Feces of adult or infant | 0/57 | 29/57 | 0-4 | 1-5 | 3-5 | 2-5 | |
| B. dentium | ATCC 15424 | Pleural fluid from adult | − | 0 | 2 | 2 | 2 | |
| ATCC 27678 | Feces of human | − | 0 | 2 | 2 | 4 | ||
| ATCC 27679 | Human vagina | − | 0 | 1 | 2 | 2 | ||
| ATCC 27680 | Human dental caries | − | 0 | 4 | 4 | 5 | ||
| ATCC 15423 | Lung abscess in adult | − | 0 | 1 | 4 | 4 | ||
| JCM 7135 | Human dental caries | − | 0 | 4 | 4 | 4 | ||
| MCC strains | Feces of infant or adult | 0/10 | 0/10 | 0 | 1-4 | 2-5 | 2-5 | |
| B. angulatum | ATCC 27535T | Feces of human | − | 0-1 | 4 | 4 | 2 | |
| ATCC 27669 | Sewage | − | 0-1 | 1 | 2 | 1 | ||
| ATCC 27671 | Sewage | − | 0-1 | 2 | 4 | 2 | ||
| B. animalis subsp. animalis | IV-58 (same as ATCC 25527T) | Feces of rat | − | 0 | 4 | 5 | 4 | |
| IV-124 (same as ATCC 27672) | Feces of rat | + | 5 | 5 | 5 | 4 | ||
| MCC strains | Feces of rat | 2/2 | 2/2 | 4-5 | 3 | 4-5 | 3-4 | |
| B. animalis subsp. lactis | IV-113 (same as ATCC 27673) | Sewage | − | 0 | 5 | 5 | 3 | |
| IV-117 (same as ATCC 27674) | Feces of rabbit | − | 0 | 5 | 5 | 5 | ||
| IV-129 (same as JCM 1253) | Feces of chicken | − | 0 | 5 | 5 | 5 | ||
| DSM 10140 | Yogurt | − | 0 | 4 | 5 | 5 | ||
| MCC strains | Yogurt | 0/3 | 0/3 | 0 | 4-5 | 4-5 | 5 | |
| B. pseudolongum | IV-70 (same as ATCC 25526T) | Feces of swine | + | 5 | 4 | 4 | 4 | |
| MCC strains | Feces of animals | 4/5 | 4/5 | 0-5 | 3-4 | 3-4 | 3-4 | |
| B. thermophilum | IV-29 (same as ATCC 25525T) | Feces of swine | − | 0 | 4 | 5 | 3 | |
| MCC strains | Feces of animals | 0/4 | 0/4 | 0 | 0-5 | 5 | 4-5 | |
These microorganisms were identified by cellular morphology, patterns of sugar fermentation, DNA homology, and species- or subspecies-specific PCR.
Number of positive strains (growth pattern category ≥ 1)/number of total strains tested.
The growth pattern was categorized from 0 to 5 based on OD660 measured at 24 h (OD24 h) and 144 h (OD144 h). 0, no growth (OD144 h < 0.1); 1, slow and mild growth (OD24 h < 0.1; OD144 h = 0.1 to 0.5); 2, slow but strong growth (OD24 h < 0.1; OD144 h > 0.5); 3, intermediate and mild growth (OD24 h = 0.1 to 0.5; OD144 h = 0.1 to 0.5); 4, intermediate and strong growth (OD24 h = 0.1 to 0.5; OD144 h > 0.5); 5, quick and strong growth (OD24 h > 0.5).