TABLE 2.
Detection of enzymatic activity of LNB phosphorylase in bifidobacterial strains
| Species | Strain(s) | PCR detection of lnpAe | Growth ond: |
Type of activityc,e |
||||
|---|---|---|---|---|---|---|---|---|
| LNB phosphorolyzinga |
LNB formingb |
|||||||
| Glucose | LNB | Glucose | LNB | Glucose | LNB | |||
| B. pseudocatenulatum | IV-152 (same as ATCC 27919T) | − | 5 | 5 | − | − | − | − |
| ATCC 27677 | − | 5 | 4 | − | − | − | − | |
| ATCC 27676 | − | 4 | 4 | − | − | − | − | |
| MCC 0310 | − | 5 | 0 | − | ND | − | ND | |
| MCC 0337 | − | 5 | 0 | − | ND | − | ND | |
| MCC 0376 | − | 5 | 0 | − | ND | − | ND | |
| B. animalis subsp. | IV-58 (same as ATCC 25527T) | − | 4 | 0 | − | ND | − | ND |
| animalis | IV-124 (same as ATCC 27672) | + | 4 | 5 | − | + | − | + |
| B. longum subsp. longum | IV-51 (same as ATCC 15707T) | + | 5 | 5 | − | + | − | + |
| B. longum subsp. infantis | IV-8 (same as ATCC 15697T) | + | 5 | 5 | − | + | − | + |
| B. breve | IV-14 (same as ATCC 15700T) | + | 5 | 5 | + | + | + | + |
| B. bifidum | IV-127 (same as ATCC 29521T) | + | 3 | 1 | + | ± | + | ± |
| B. pseudolongum | IV-70 (same as ATCC 25526T) | + | 4 | 5 | − | + | − | + |
a
LNB-phosphorolyzing activity was indicated by the detection of Gal1P degraded from LNB in the presence of phosphate by TLC after incubation for 5 to 18 h.
b
LNB-forming activity was indicated by the detection of LNB formation from Gal1P and GlcNAc by TLC after incubation for 24 to 66 h.
c
LNB and glucose were the carbohydrate sources used in cultivating each strain for the preparation of crude cell extract.
d
Growth patterns as described in Table 1.
e
+, presence of lnpA or enzymatic activity; −, absence of lnpA or enzymatic activity; ±, weak activity; ND, not examined. Crude cell extract boiled for 10 min showed no activity (data not shown).