. 2009 Oct 30;76(1):203–211. doi: 10.1128/AEM.01921-09

TABLE 1.

Primers and probes preloaded in the GeneDisc

Target gene sequence^a	Forward primer, reverse primer, and probe sequences (5′-3′)^b	Location within sequence	GenBank accession no.	Reference
stx₁	`TTT GTY ACT GTS ACA GCW GAA GCY TTA CG`	878-906	M16625	28
	`CCC CAG TTC ARW GTR AGR TCM ACR TC*`	983-1008
	`ROX- CTG GAT GAT CTC AGT GGG CGT TCT TAT GTA A-BHQ`	941-971
stx₂	`TTT GTY ACT GTS ACA GCW GAA GCY TTA CG`	785-813	X07865	28
	`CCC CAG TTC ARW GTR AGR TCM ACR TC*`	887-912
	`FAM- TCG TCA GGC ACT GTC TGA AAC TGC TCC-BHQ`	838-864
eae	`CAT TGA TCA GGA TTT TTC TGG TGA TA`	899-924	Z11541	26
	`CTC ATG CGG AAA TAG CCG TTA*`	979-1000
	`ROX- AT AGT CTC GCC AGT ATT CGC CAC CAA TAC-BHQ*`	936-966
ehxA	`GTG TCA GTA GGG AAG CGA ACA`	41832-41852	AF074613	This study
	`ATC ATG TTT TCC GCC AAT G*`	41939-41957
	`FAM- CGT GAT TTT GAA TTC AGA ACC GGT GG-BHQ`	41868-41893
ent/espL2	`TCC TGG ATT ATT TTC TGC ATT TCA`	3929758-3929781	AE005174	This study
	`ACT ATT GCC AAG TAC GCC ACA A*`	3929833-3929812
	`FAM-AAT GGT CAT GCA GAC GCA ATA AAG GCA TA-BHQ`	3929783-3929811
nleB	`CAT GTT GAA GGC TGG AAS TTT GT`	3931502-3931524	AE005174	This study
	`CCG CTA CAG GGC GAT ATG TT*`	3931573-3931554
	`FAM-ACA GAG ACG GGA AAA ACT GGA TGC CA-BHQ`	3931527-3931552
nleE	`AGA AGC GTT TGA ACC TAT TTC CA`	3932207-3932229	AE005174	This study
	`TTG GGC GTT TTC CGG ATA T*`	3932289-3932271
	`FAM-AGC CAG TAC ACC GGA AGG AAG CTG G-BHQ`	3932237-3932261
nleF	`TGA GGT GAG AAA TGA AAA TAC TGA TG*`	2281256-2281231	AE005174	This study
	`CTA TCC CTG TCC TCT ATC GTC ATTC`	2281182-2281206
	`ROX-TGT CGG AGC GCT GAG GGC G-BHQ*`	2281226-2281208
nleH1-2	`ACA AGA GAA AGT CAT AGT GGT TG`	2282298-2282276	AE005174	This study
	`AAT CTC YCC CTT AGG CCA TCC CA*`	2282230-2282252
	`ROX-TTT ACT AAT CTG TTG CAC AGG-BHQ`	2282274-2282254
nleA	`AGA TAA CYC TAA TAC TAA ATA TGC C`	2285138-2285162	AE005174	This study
	`GCC CAA CCA TTG CRC CGA TAT GAG G*`	2285274-2285250
	`ROX-TTC TTA CCA ATG CTG CCG CAA ATG CGC-BHQ`	2285164-2285190

stx₁, gene encoding Shiga toxin 1; stx₂, gene encoding Shiga toxin 2; eae, gene encoding intimin; ehxA, gene encoding enterohemolysin; ent/espL2, gene encoding the putative non-LEE effector ent/espL2; nleB, gene encoding the putative non-LEE effector B; nleE, gene encoding the putative non-LEE effector E; nleF, gene encoding the putative non-LEE effector F; nleH1-2, gene encoding the putative non-LEE effector H1-2; nleA, gene encoding the putative non-LEE effector A.

In the sequence of oligonucleotides: Y, (C, T); S, (C, G); W, (A, T); R, (A, G); M, (A, C); K, (G, T); H, (A, T, C); D, (G, A, T). FAM, 6-carboxylfluorescein; ROX, carboxy-X-rhodamine; BHQ, black hole quencher; *, complementary strand.