TABLE 2.
Virulotyping of the eae and nle genes in EHEC strains
Serotype | No. tested | stx pattern | Virulotype |
|||||||
---|---|---|---|---|---|---|---|---|---|---|
ehxA | eae | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA | |||
Or[157]:H7, O157:[H7]a | 68b,c,d | 3 stx1, 43 stx2, 22 stx1 and stx2 | ehxA | gamma | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O157:[H7] | 6b,c,d | 1 stx1, 4 stx2, 1 stx1 and stx2 | ehxA | gamma | ent/espL2 | nleB | nleE | nleH1-2 | nleA | |
O157:[H7] | 2b | 2 stx2 | ehxA | gamma | ent/espL2 | nleB | nleE | nleH1-2 | ||
O103:H2 | 23b,c,d | 21 stx1, 1 stx2, 1 stx1 and stx2 | ehxA | epsilon | ent/espL2 | nleB | nleE | nleH1-2 | ||
O103:H2 | 2b,d | 2 stx1 | epsilon | ent/espL2 | nleB | nleE | nleH1-2 | |||
O103:[H2] | 1 | stx1 | ehxA | ent/espL2 | nleB | nleE | nleH1-2 | |||
O111:[H8] | 21b,d | 13 stx1, 3 stx2, 5 stx1 and stx2 | ehxA | theta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O111:[H−] | 2b | 1 stx2, 1 stx1 and stx2 | ehxA | theta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O111:H8 | 1b | stx1 | theta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA | |
O111:H8 | 1b | stx1 and stx2 | theta | ent/espL2 | nleB | nleE | nleH1-2 | nleA | ||
O26:[H11] | 22b,c,d | 12 stx1, 7 stx2, 3 stx1 and stx2 | ehxA | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O26:[H11] | 7b | 2 stx1, 3 stx2, 2 stx1 and stx2 | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA | |
O26:[H11] | 3b,d | 2 stx1, 1 stx1 and stx2 | ehxA | beta | ent/espL2 | nleB | nleE | nleH1-2 | nleA | |
O26:[H11] | 1b | stx1 | beta | ent/espL2 | nleB | nleE | nleH1-2 | nleA | ||
O26:H11 | 1b | stx1 | ehxA | beta | ent/espL2 | nleB | nleE | nleA | ||
O145:[H28] | 17b,d | 8 stx1, 8 stx2, 1 stx1 and stx2 | ehxA | gamma | ent/espL2 | nleB | nleE | nleH1-2 | ||
O145:H28 | 1b | stx1 | ehxA | gamma | ent/espL2 | nleB | nleE | nleH1-2 | nleA | |
O121:H19 | 4b | 4 stx2 | ehxA | epsilon | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O5:H− | 12b,c | 12 stx1 | ehxA | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O103:H25 | 1b | stx2 | ehxA | theta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O55:H7 | 2b,c | 2 stx2 | gamma | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA | |
O118:H16 | 19b,d | 16 stx1, 3 stx1 and stx2 | ehxA | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O118:H16 | 2b | 2 stx1 | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA | |
O123:H11 | 1b | stx2 | ehxA | beta | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O165:H25 | 1b | stx2 | ehxA | epsilon | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O172:[H25] | 1b | stx2 | ehxA | epsilon | ent/espL2 | nleB | nleE | nleF | nleH1-2 | nleA |
O45:H2 | 1b | stx1 | ehxA | epsilon | ent/espL2 | nleB | nleE | nleH1-2 | ||
O15:H2 | 1b | stx2 | beta | ent/espL2 | nleB | nleE | nleH1-2 |
Six of these were SF O157:H− and two were O-rough:[H7] positive for the rfbEO157 gene. Brackets indicate genotyping of the flic or rfb genes.
Clinical isolate.
Food isolate.
Animal/environment isolate.