FIG. 2.

Targeted disruption of the BbJEN1 locus. (A) BbJEN1 locus and disruption vector (pBFT-JEN1L/R). pBFT-JEN1L/R, containing the bar::egfp cassette, was digested with HindIII and purified by gel electrophoresis to transform B. bassiana Bb0062 conidia. (B) DNA gel blot analysis of BbJEN1-disrupted strains. Genomic DNA was digested with NcoI/SmaI (up) and DraI (down). The 550-bp BbJEN1 fragment (up) and the 500-bp bar fragment (down) were labeled with [α-³²P]dCTP and used as a hybridization probe. E-22 is an ectopic transformant. “WT” represents the wild-type strain. ΔJEN1-15, ΔJEN1-20, ΔJEN1-24, and ΔJEN1-212 are BbJEN1-disrupted strains.