FIG. 3.

Overexpression of BbJEN1 in B. bassiana. Northern blot analysis was performed by probing total RNA of the B. bassiana wild-type strain and transformant hyphae grown in SDY (containing 4% [wt/vol] glucose to repress the expression of the native BbJEN1 gene) with the 550-bp BbJEN1 fragment labeled by [α-³²P]dCTP. Each lane represents about 30 μg of total RNA. The bottom panel shows the loading controls, where each lane represents 20 μg of total RNA. “WT” represents the wild-type strain. T-4 is a transgenic control. Ov-30 and Ov-33 are BbJEN1 overexpression strains.