FIG. 4.

BbJEN1 could transport the carboxylates in B. bassiana. Hyphae were cultivated for about 2 days in SDY medium and then harvested and transferred to volumes of minimal medium supplemented with different carboxylates at concentrations of 0.2% and were incubated for 24 h. The amounts of carboxylates in hyphae were measured with capillary electrophoresis. “WT” represents the wild-type strain. T-4 is a transgenic control. Ov-30 and Ov-33 are BbJEN1-overexpression strains. E-22 is an ectopic transformant. ΔJEN1-20 and ΔJEN1-212 are BbJEN1-disrupted strains.