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GFP fluorescence was induced by carboxylates and cicada slough and repressed by glucose in the BbJEN1p::egfp line. Hyphae were cultivated for 48 h in SDY and transferred to volumes of minimal medium containing various substrates. After being cultivated for 12 h, hyphae were observed with epifluorescence or bright-field microscopy. Bars = 25 μm.
