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. 2009 Dec 14;120(1):331–342. doi: 10.1172/JCI38702

Figure 3. Immunofluorescent labeling demonstrates recruitment of 7/4+ neutrophils and CXCL1 expression in kidneys following IRI.

Figure 3

(A–D) Panoramic views of kidney through the entire extent of cortex (lateral, left) and medulla (medial, right) were generated by stitching together 4 overlapping images (Adobe Photoshop). Labels at the top delineating approximate boundaries of cortex and inner and outer medulla apply to A and B (C and D are at a slightly higher magnification). (A) A large influx of 7/4+ neutrophils (FITC epifluorescence; green) was seen in the kidney outer medulla 24 hours after IRI in WT mice. Some CXCL1 immunoreactivity (Cy3 epifluorescence; red) was evident in medullary tubule cells, but most was associated with infiltrating neutrophils. (B–D) Significantly less neutrophil recruitment and CXCL1 expression were observed in p19–/–, Il17r–/–, and Il17a–/– IRI mouse kidneys. (E–J) Higher magnification of images shows substantial neutrophil infiltration and colocalization (yellow) of CXCL1 and 7/4 immunoreactivity in neutrophils in medulla of WT IRI kidney (E and F) but not in kidneys from the KO mice that were protected from injury (G–J). Nuclei were labeled with DAPI (blue). Scale bars: 100 μm (A–D); 10 μm (F); 40 μm (E, G–J).