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. 2009 Dec 1;120(1):290–302. doi: 10.1172/JCI39194

Figure 3. Increased Hdm2 in response to TGF-β1 is independent of p53.

Figure 3

(A) 293T and SKOV3 cells were untreated (UT) or treated with vehicle or TGF-β1 (10 ng/ml) for 24 and 48 hours. Cellular extracts were prepared for Western blot analysis of Hdm2 and Ku70. (B) SKOV3 and 293T cells were transiently transfected with 0, 4, or 10 μg of constitutively active TβRI (caTβRI) expression plasmid. Cellular extracts were prepared for Western blot analysis of Hdm2 and α-tubulin. (C) Transient transfection of 293T, SKOV3, and HCT116:3-6 cells with the caTβRI and HDM2 reporter or the SBE2X2 reporter construct. All samples were transfected with a renilla construct. Reporter activity was determined relative to renilla to generate relative activity. Fold induction was determined relative to vehicle control and SD was calculated relative to the mean.