Figure 1. Effects of β cell–specific ablation of C/EBPβ on glucose metabolism and islet morphology.

(A) Pancreatic islets isolated from 24-week-old Cebpb^fl/fl control or βCebpb^–/– mice were lysed and subjected to immunoblot analysis with antibodies to C/EBPβ and to β-actin (loading control). (B and C) Fed blood glucose (B) and plasma insulin (C) concentrations of control (open circles) or βCebpb^–/– (filled circles) mice at the indicated ages. Data are mean ± SEM from at least 6 mice per genotype. (D) Immunostaining of pancreatic sections from 24-week-old control or βCebpb^–/– mice with antibodies to insulin (red) and to glucagon (green). Scale bars: 100 μm.