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. 2009 Dec 21;120(1):254–265. doi: 10.1172/JCI40295

Figure 5. KLF5 controls expression of paracrine factors in cardiac fibroblasts that mediate cardiomyocyte hypertrophy.

Figure 5

(A) siRNA-mediated knockdown of Klf5 in cardiac fibroblasts. Klf5 levels were normalized to those in cells transfected with the control siRNA (siCntrl). *P < 0.01 versus siCntrl. (B–D) Cultured cardiomyocytes were incubated with serum-free medium (SFM) or conditioned medium prepared from cardiac fibroblasts transfected with control or Klf5 siRNA for 48 hours. (B) Representative cardiomyocytes are shown stained for sarcomeric α-actinin (green) and nuclei (Hoechst 33258, blue). Scale bar: 10 μm. (C) Cell surface areas of 100 cells from each group. *P < 0.01 versus cells treated with SFM; #P < 0.05 versus cells treated with medium conditioned by siCntrl transfectants. (D) ANP concentrations in culture medium conditioned by cardiomyocytes. *P < 0.05 versus SFM; #P < 0.05 versus siCntrl.