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. 2010 Jan 5;5(1):e8581. doi: 10.1371/journal.pone.0008581

Figure 5. Chk1+/− erythroid progenitors show a marked increase in spontaneous DNA damage foci.

Figure 5

Progenitors from stages I–IV were sorted from WT and Chk1+/− mice and immunostained with DNA damage markers 53BP1(green), pH2AX(red) and DAPI(blue). The Chk1+/− erythroid progenitors from stages I–IV showed dramatic increase and co-localization of 53BP1 and pH2AX at the site of spontaneous DNA damage and double strand break in the nucleus, resulting in miscoordination of enucleation during erythropoiesis compared to WT counterparts at 60× magnification and images scaled at 20 µm.