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The effect of GCH-1 phosphorylation mutation on subcellular GCH-1 activity. The cytoplasmic and nuclear GCH-1 activity for cells expressing WT-GCH-1 or its phospho-defective mutants were shown in (A) and (B) respectively. N=3, #=p<0.05 vs. GCH-1 cells without tetracycline;*=p<0.05 and NS= not significant vs. GCH-1 with tetracycline.
