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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Proteome Res. 2009 Mar;8(3):1415–1422. doi: 10.1021/pr800774h

Figure 1.

Figure 1

Two fragmentation spectra produced on a QSTAR Pulsar I from bovine serum albumin. The peptides produced by Lys-N digestion (A) and trypsin digestion (B) differ by a single amino acid. The two peptides produce reciprocal long y- and b-type ion series, respectively. The immonium ion peaks (pink) originate from the end of the peptide with the least basicity; the carboxy terminal end of the peptide in the case of the Lys-N peptide, and the amino terminal end in the case of the tryptic peptide.