. 2010 Jan 8;6(1):e1000716. doi: 10.1371/journal.ppat.1000716

Table 1. Bacterial strains and plasmids used in this study.

Strains/Plasmids	Relevant characteristics^a	Reference or source
Strains
E. faecalis
OG1RF	Laboratory strain; Rif ^r, Fus^r, Chl^s, Gen^s, Kan^s	[61]
TX5256	ace insertion disruption mutant of OG1RF; Rif ^r, Fus^r, Kan^r, Chl^s, Gen^s	[11]
TX5467	OG1RFΔace::cat, ace deletion mutant of OG1RF; Rif ^r, Fus^r, Kan^s, Chl^r, Gen^s	This study
TX5647	TX5467 harboring pTEX5646 (for complementation with the ace gene); Rif ^r, Fus^r, Chl^r, Gen^r	This study
TX5648	TX5467 harboring pAT392 (control for complementation); Rif ^r, Fus^r, Chl^r, Gen^r	This study
E. coli
DH5α	E. coli host strain for routine cloning	Stratagene
XL1-Blue	E. coli host strain for routine cloning	Stratagene
LMG194	E. coli strain for expression of recombinant proteins	Invitrogen
TX5254	LMG194 (pBAD::ace); 1008 bp OG1RF ace (coding for complete A domain) cloned into pBAD/HisA expression vector; Amp^r	[11]
TX5428	DH5α (pTEX5428); Chl^r, Gen^r	This study
TX5646	XL1-Blue (pTEX5646); Gen^r	This study
Plasmids
pAT392	Shuttle expression vector (Gen^r Spc^r oriR _pUC oriR _pAMβ1 oriT _RK2 P₂)	[51]
pTEX5500ts	Shuttle plasmid, ts in Gram⁺ hosts; Chl^r, Gen^r	[50]
pTEX5646	Construct for complementation; a 2,186-bp fragment containing ace cloned into pAT392 downstream of the P2 promoter	This study
pTEX5428	Plasmid for ace deletion with flanking regions of the ace gene cloned on either side of the cat gene into pTEX5500ts; Chl^r, Gen^r	This study

^aChl, chloramphenicol; Fus, fusidic acid; Gen, gentamicin; Kan, kanamycin; Rif, rifampicin; and ts, temperature-sensitive. Superscript “s” designates sensitivity and “r” designates resistance; “r” is defined for enterococci as MIC >500 for Gen and >2000 for Kan.