Table 2. Primers used.
Target | Upstream primer (5′ → 3′) | Downstream primer (5′ → 3′) |
mmp14 | CCCAGATAAGCCCAAAAACC | GCATTGGGTATCCATCCATC |
irg1 | TTCCAAGCCTGGATTCTCAC | TCACAAGGTGCTTTCTCACG |
ifit2 | TACCCATCAGCAAGATGCAC | CTGTGTCAAAGCGCTCAAAG |
arg2 | TGGATCAAACCTTGCCTCTC | GCCGATCAAATGTCTGTTCC |
ifi44 | GCATATGCTGCATTGTCACC | TCCATTCCCAGTCCTTTCAG |
ccl22 | TCCCAGGGGAAGGAATAAAC | TTGTGGTCCCATATGCTGTC |
ifit1 | ATGGGAGAGAATGCTGATGG | CCCAATGGGTTCTTGATGTC |
olr1 | TGGCTATGGGAGAATGGAAC | CAGTTTTCAGCGAACACAGC |
nfkbie | AAGGATTGCAGACGAGGAAG | ATCCCCATTTTCCAGGTAGC |
ccl17 | AAAGGGGCCATTCCTATCAG | CCAATCTGATGGCCTTCTTC |
mx2 | AGACAAAGCATGGCACTTCC | ACTGGATGATCAAGGGAACG |
mx1 | TGCTCATCTCCGACTGTTTG | TGGGGTACACAGGTGAAATG |
Ifnb1 | CCCTATGGAGATGACGGAGA | TCCCACGTCAATCTTTCCTC |
actb | TGGAATCCTGTGGCATCCATGAAAC | TAAAACGCAGCTCAGTAACAGTCCG |
These primers were designed using the primer3 tool available at the following website: http://frodo.wi.mit.edu/.